Mouse Calcium Channel, Voltage Dependent, T-Type, Alpha 1H Subunit (CACNa1H) ELISA Kit

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SKU:
MODL00196
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
O88427
Sensitivity:
0.117ng/mL
Range:
0.312-20ng/mL
ELISA Type:
Sandwich
Synonyms:
CACNA1HB,Cav3.2,Low-voltage-activated calcium channel alpha1 3.2 subunit,Voltage-gated calcium channel subunit alpha Cav3.2
Reactivity:
Mouse
Sample Type:
Tissue Homogenates And Other Biological Fluids
Frequently bought together:

Description

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Mouse Calcium Channel, Voltage Dependent, T-Type, Alpha 1H Subunit (CACNa1H) ELISA Kit

The Mouse Calcium Channel Voltage-Dependent T-Type Alpha-1H Subunit (CACNA1H) ELISA Kit is a powerful tool for accurately measuring levels of this important protein in mouse samples. This kit offers high sensitivity and specificity, ensuring precise and consistent results for a variety of research applications.CACNA1H is a key component of calcium channels involved in regulating cell membrane potential and calcium influx. Dysregulation of these channels has been implicated in various diseases, including neurological disorders and cardiac conditions.

By accurately measuring CACNA1H levels, researchers can gain valuable insights into disease mechanisms and potential therapeutic targets.Whether studying the role of CACNA1H in neurobiology, cardiovascular health, or other areas of research, the Mouse Calcium Channel Voltage-Dependent T-Type Alpha-1H Subunit ELISA Kit provides a reliable and efficient means of quantifying this important protein.

Product Name:Mouse Calcium Channel, Voltage Dependent, T-Type, Alpha 1H Subunit (CACNa1H) ELISA Kit
Product Code:MODL00196
Size:96 Assays
Target:CACNa1H
Synonyms:CACNA1HB, Cav3.2, Low-voltage-activated calcium channel alpha1 3.2 subunit, Voltage-gated calcium channel subunit alpha Cav3.2
Detection Method:Sandwich
Range:0.312-20ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)83-10091
EDTA plasma (n=5)90-9894
Heparin plasma (n=5)89-10295
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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