The Mouse Calcineurin CAN ELISA Kit is a powerful tool for the precise measurement of calcineurin levels in mouse serum, plasma, and cell culture supernatants. With its outstanding sensitivity and specificity, this kit delivers results that are accurate and reproducible, making it the perfect choice for a variety of research applications.Calcineurin is a key enzyme that plays a critical role in regulating various cellular processes, including immune responses, nerve cell function, and muscle contraction. Dysregulation of calcineurin activity has been linked to conditions such as autoimmune diseases, neurodegenerative disorders, and cardiac hypertrophy, highlighting its importance as a potential therapeutic target.
By accurately measuring calcineurin levels, researchers can gain valuable insights into the underlying mechanisms of these diseases and develop targeted interventions to effectively modulate calcineurin activity. The Mouse Calcineurin CAN ELISA Kit is a reliable and efficient tool for advancing our understanding of calcineurin biology and its implications for human health.
Product Name:
Mouse Calcineurin (CaN) ELISA Kit
Product Code:
MODL00199
Size:
96 Assays
Target:
CaN
Synonyms:
PPP3CA, PPP3-CA, CCN1, CNA1, CALN, CALNA1, PP2B, PP2-B, Protein Phosphatase 3(formerly 2B),Catalytic Subunit,Alpha Isoformprotein Phosphatase 2B, Calcineurin A
Detection Method:
Sandwich
Range:
0.312-20ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-99
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
