Mouse C5a Recombinant Protein (RPPB2951)
- SKU:
- RPPB2951
- Product type:
- Recombinant Protein
- Size:
- 10ug
- Species:
- Mouse
- Target:
- C5a
- Synonyms:
- Complement C5
- Hemolytic complement
- C5
- Hc
- Source:
- Escherichia Coli
- Uniprot:
- P06684
Description
| Product Name: | Mouse C5a Recombinant Protein |
| Product Code: | RPPB2951 |
| Size: | 10µg |
| Species: | Mouse |
| Target: | C5a |
| Synonyms: | Complement C5, Hemolytic complement, C5, Hc, He, C5a. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation: | Mouse C5a was lyophilized from a concentrated (1mg/ml) solution in 20mM PB, pH 7.5 and 350mM NaCl. |
| Solubility: | It is recommended to reconstitute the lyophilized Mouse C5a in sterile 18M?-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions. |
| Stability: | Lyophilized Mouse C5a although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Mouse C5a should be stored at 4°C between 2-7 days and for future use below -18°C.Please prevent freeze-thaw cycles. |
| Purity: | Greater than 95.0% as determined by:(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE. |
| Amino Acid Sequence: | The sequence of the first five N-terminal amino acids was determined and was found to be Asn-Leu-His-Leu-Leu |
| Biological Activity: | The ED50 of Recombinant Mouse C5a as determined by its ability to induce N-acetyl-b-D-glucosaminidase release from differentiated U937 human histiocytic lymphoma cells was 5-20ng/ml. |
Mouse Complement 5a (C5a) is a glycoprotein which is a member of a family of structurally and functionally related proteins known as anaphylatoxins. C5a functions through G-protein coupled receptor (GPCR) (C5aR/CD88). C5a is a effective chemoattractant and anaphylatoxin which functions on all classes of leukocytes and on many other cell types including endothelial, smooth muscle, kidney, liver, and neural cells. Mouse C5a also mediates IL-8 release from bronchial epithelial cells. Furthermore, it triggers an oxidative surge in macrophages and neutrophils, causing the release of histamine in basophils and mast cells. The C5a anaphylatoxin activity on hepatocytes results indirectly from interaction with nonparenchymal cell via prostanoid secretion.
C5a Mouse Recombinant produced in E.Coli is a single, non-glycosylated, Polypeptide chain containing 77 amino acids and having a molecular mass of 9kDa.The Mouse C5a is purified by proprietary chromatographic techniques.
| UniProt Protein Function: | C5: Activation of C5 by a C5 convertase initiates the spontaneous assembly of the late complement components, C5-C9, into the membrane attack complex. C5b has a transient binding site for C6. The C5b-C6 complex is the foundation upon which the lytic complex is assembled. Defects in C5 are the cause of complement component 5 deficiency (C5D). A rare defect of the complement classical pathway associated with susceptibility to severe recurrent infections, predominantly by Neisseria gonorrhoeae or Neisseria meningitidis. An association study of C5 haplotypes and genotypes in individuals with chronic hepatitis C virus infection shows that individuals homozygous for the C5_1 haplotype have a significantly higher stage of liver fibrosis than individuals carrying at least 1 other allele (PubMed:15995705). |
| UniProt Protein Details: | Protein type:Secreted; Secreted, signal peptide Cellular Component: membrane attack complex; extracellular space; extracellular region Molecular Function:endopeptidase inhibitor activity Biological Process: positive regulation of angiogenesis; in utero embryonic development; immune system process; cytolysis; complement activation, alternative pathway; innate immune response; inflammatory response; complement activation, classical pathway |
| UniProt Code: | P06684 |
| NCBI GenInfo Identifier: | 116608 |
| NCBI Gene ID: | 15139 |
| NCBI Accession: | P06684.2 |
| UniProt Secondary Accession: | P06684,A2AS36, |
| UniProt Related Accession: | P06684 |
| Molecular Weight: | 188,878 Da |
| NCBI Full Name: | Complement C5 |
| NCBI Synonym Full Names: | hemolytic complement |
| NCBI Official Symbol: | Hc |
| NCBI Official Synonym Symbols: | C5; He; C5a |
| NCBI Protein Information: | complement C5 |
| UniProt Protein Name: | Complement C5 |
| UniProt Synonym Protein Names: | Hemolytic complementCleaved into the following 4 chains:Complement C5 beta chain; Complement C5 alpha chain; C5a anaphylatoxin; Complement C5 alpha' chain |
| Protein Family: | Complement C5 |
| UniProt Gene Name: | C5 |
| UniProt Entry Name: | CO5_MOUSE |
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