The Mouse C3a Complement Component 3a ELISA Kit is specifically designed to accurately measure levels of C3a complement component 3a in mouse serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and reproducible results for a variety of research purposes.C3a complement component 3a is a key player in the complement system, a crucial component of the immune response that helps to clear pathogens and promote inflammation. Dysregulation of the complement system has been linked to various diseases, including autoimmune disorders, infections, and inflammatory conditions.
Therefore, the measurement of C3a levels is important for understanding the role of this protein in health and disease, as well as for the development of potential therapeutic interventions.Overall, the Mouse C3a Complement Component 3a ELISA Kit from Assay Genie provides researchers with a valuable tool for investigating the function and regulation of C3a in mouse models, offering reliable and accurate results for a wide range of experimental settings.
Product Name:
Mouse C3a (Complement Component 3a) ELISA Kit
SKU:
MOES00900
Target:
Mouse C3a (Complement Component 3a)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.10 ng/mL
Detection range:
0.16-10 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse C3a. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse C3a and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse C3a, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse C3a. You can calculate the concentration of Mouse C3a in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
98-112
96-109
89-102
Average (%)
106
103
96
1:4
Range (%)
88-99
84-97
89-101
Average (%)
94
89
94
1:8
Range (%)
92-105
83-98
83-95
Average (%)
97
90
89
1:16
Range (%)
90-104
84-95
86-102
Average (%)
97
90
93
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
91-107
98
EDTA plasma (n=5)
95-109
100
Cell culture media (n=5)
91-107
98
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.47
1.27
4.69
0.5
1.15
5.0
Standard deviation
0.03
0.07
0.2
0.03
0.06
0.22
C V (%)
6.38
5.51
4.26
6.0
5.22
4.4
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Mouse C3a concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse C3a in samples. No significant cross-reactivity or interference between Mouse C3a and analogues was observed.
