The Mouse C-P-C Peptide ELISA Kit is specifically designed for the accurate detection of C-P-C peptide levels in mouse serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, guaranteeing reliable and consistent results for a variety of research applications.C-P-C peptide is an important protein involved in a range of physiological processes, including cell communication, regulation of gene expression, and immune response modulation.
Its levels are implicated in various diseases, such as diabetes, obesity, and inflammation, making it a valuable biomarker for studying these conditions and exploring potential treatments.Overall, the Mouse C-P-C Peptide ELISA Kit provides researchers with a powerful tool for investigating the role of C-P-C peptide in health and disease, offering precise and accurate measurements for insightful research findings.
Product Name:
Mouse C-P (C-Peptide) ELISA Kit
SKU:
MOES00913
Target:
Mouse C-P (C-Peptide)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.10 ng/mL
Detection range:
0.16-10 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse C-P. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse C-P and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse C-P, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse C-P. You can calculate the concentration of Mouse C-P in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
99-112
89-103
97-112
Average (%)
105
95
105
1:4
Range (%)
98-112
84-97
93-108
Average (%)
106
89
101
1:8
Range (%)
100-113
85-95
98-113
Average (%)
105
90
104
1:16
Range (%)
101-114
85-98
96-110
Average (%)
108
90
103
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
89-102
94
EDTA plasma (n=5)
87-97
92
Cell culture media (n=5)
90-105
97
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.5
1.4
3.4
0.5
1.4
3.3
Standard deviation
0.03
0.08
0.15
0.03
0.08
0.16
C V (%)
6
5.71
4.41
6.0
5.71
4.85
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Mouse C-P concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse C-P in samples. No significant cross-reactivity or interference between Mouse C-P and analogues was observed.
