Mouse BRAK (Breast And Kidney Expressed Chemokine) ELISA Kit
The Mouse BRAK (Breast and Kidney expressed Chemokine) ELISA Kit is specifically designed for the precise measurement of BRAK levels in mouse serum, plasma, and cell culture supernatants. This cutting-edge kit boasts exceptional sensitivity and accuracy, ensuring dependable and consistent results for a variety of research purposes.BRAK is a key chemokine involved in inflammatory responses and immune regulation, playing a critical role in various physiological and pathological processes.
It is a valuable biomarker for studying conditions such as cancer, inflammation, and autoimmune diseases, offering insights into disease mechanisms and potential therapeutic interventions.Unlock the potential of your research with the Mouse BRAK ELISA Kit, providing the tools needed to explore the complex role of this essential chemokine in biological systems.
Product Name:
Mouse BRAK (Breast And Kidney Expressed Chemokine) ELISA Kit
SKU:
MOES00784
Target:
Mouse BRAK (Breast And Kidney Expressed Chemokine)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.10 ng/mL
Detection range:
0.16-10 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse BRAK/CXCL14. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse BRAK/CXCL14 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse BRAK/CXCL14, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse BRAK/CXCL14. You can calculate the concentration of Mouse BRAK/CXCL14 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
94-107
94-107
95-107
Average (%)
100
102
101
1:4
Range (%)
101-118
84-96
93-108
Average (%)
107
90
99
1:8
Range (%)
96-111
87-101
90-107
Average (%)
103
92
98
1:16
Range (%)
95-110
85-97
96-112
Average (%)
103
92
102
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
95-112
102
EDTA plasma (n=5)
93-107
99
Cell culture media (n=5)
94-111
102
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.5
1.3
3.8
0.5
1.4
3.6
Standard deviation
0.03
0.07
0.13
0.03
0.08
0.18
C V (%)
6
5.38
3.42
6.0
5.71
5.0
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Mouse BRAK/CXCL14 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse BRAK/CXCL14 in samples. No significant cross-reactivity or interference between Mouse BRAK/CXCL14 and analogues was observed.
