The Mouse BLC (B Lymphocyte Chemoattractant) ELISA Kit is specially designed for the accurate and precise measurement of mouse BLC levels in serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit delivers reliable and reproducible results, making it an essential tool for various research applications.BLC, also known as CXCL13, is a key chemokine involved in the recruitment and migration of B lymphocytes to lymphoid organs, where they play a crucial role in immune responses.
Dysregulation of BLC levels has been implicated in various immune-related disorders, making it a valuable biomarker for studying the immune system and developing potential therapeutic interventions.With the Mouse BLC ELISA Kit, researchers can accurately quantify BLC levels in their samples, enabling them to better understand the mechanisms underlying immune responses and potentially identify novel targets for therapeutic intervention.
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse BLC. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse BLC and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse BLC, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse BLC. You can calculate the concentration of Mouse BLC in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
87-99
95-105
88-104
Average (%)
93
100
96
1:4
Range (%)
91-107
83-99
83-98
Average (%)
98
90
89
1:8
Range (%)
88-102
80-91
87-100
Average (%)
94
86
93
1:16
Range (%)
91-105
80-93
90-102
Average (%)
97
86
95
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
87-102
93
EDTA plasma (n=5)
91-103
96
Cell culture media (n=5)
89-100
95
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
51.22
89.77
396.03
48.37
85.44
408.04
Standard deviation
3.45
4.27
15.52
3.08
4.21
14.77
C V (%)
6.74
4.76
3.92
6.37
4.93
3.62
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Mouse BLC concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse BLC in samples. No significant cross-reactivity or interference between Mouse BLC and analogues was observed.
