Mouse Bid (BH3 Interacting Domain Death Agonist) CLIA Kit
The Mouse BID (BH3-interacting domain death agonist) CLIA Kit is specifically designed for the precise detection of BID levels in mouse serum, plasma, and cell lysates. This kit offers exceptional sensitivity and specificity, ensuring accurate and consistent results for a variety of research purposes. BID is a critical protein involved in apoptosis, playing a key role in regulating cell death pathways. Dysregulation of BID has been associated with various diseases, including cancer, neurodegenerative disorders, and autoimmune conditions.
This makes it a valuable biomarker for studying disease mechanisms and exploring potential therapeutic interventions. The Mouse BID CLIA Kit from Assay Genie provides researchers with a reliable and efficient tool for investigating the role of BID in disease pathology and developing targeted treatments. Trust in the accuracy and precision of this kit for your research needs.
Product Name:
Mouse Bid (BH3 Interacting Domain Death Agonist) CLIA Kit
SKU:
MOES00118
Target:
Mouse Bid (BH3 Interacting Domain Death Agonist)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse Bid. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse Bid and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse Bid, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse Bid. You can calculate the concentration of Mouse Bid in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
95-110
97-110
90-102
Average (%)
102
102
97
1:4
Range (%)
85-99
92-105
96-107
Average (%)
91
98
101
1:8
Range (%)
88-99
87-100
101-115
Average (%)
93
95
107
1:16
Range (%)
94-108
94-108
90-103
Average (%)
99
100
96
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
100-114
108
EDTA plasma (n=5)
91-104
97
Cell culture media (n=5)
93-105
100
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
184.23
469.21
1620.37
179.54
440.88
1509.99
Standard deviation
16.4
50.49
185.21
17.49
46.69
100.11
C V (%)
8.9
10.76
11.43
9.74
10.59
6.63
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse Bid concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse Bid in samples. No significant cross-reactivity or interference between Mouse Bid and analogues was observed.
