Mouse BH3 Interacting Domain Death Agonist (Bid) ELISA Kit
The Mouse BH3 Interacting Domain Death Agonist (BID) ELISA Kit is a specialized tool for the precise measurement of BID levels in mouse serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and consistent results for various research purposes.BID is a key protein involved in apoptosis regulation and cell death pathways, making it a crucial player in various physiological and pathological processes. By accurately measuring BID levels, researchers can gain valuable insights into the mechanisms underlying diseases such as cancer, neurodegenerative disorders, and autoimmune conditions, paving the way for novel therapeutic interventions.
Overall, the Mouse BH3 Interacting Domain Death Agonist (BID) ELISA Kit is an indispensable tool for researchers seeking to unravel the intricacies of cell death pathways and explore potential therapeutic targets in a mouse model system.
Product Name:
Mouse BH3 Interacting Domain Death Agonist (Bid) ELISA Kit
Product Code:
MODL00154
Size:
96 Assays
Target:
Bid
Synonyms:
p22 BID
Detection Method:
Sandwich
Range:
0.312-20ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
86-100
93
EDTA plasma (n=5)
87-100
93
Heparin plasma (n=5)
88-100
94
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
