The Mouse Beta TG (Beta Thromboglobulin) ELISA Kit is specifically designed for the precise measurement of beta thromboglobulin levels in mouse serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit ensures accurate and reproducible results, making it an excellent choice for a variety of research applications.Beta thromboglobulin is a key protein involved in platelet activation and aggregation, playing a crucial role in the process of blood clot formation.
Abnormal levels of beta thromboglobulin have been associated with various thrombotic disorders, making it a valuable biomarker for studying these conditions and developing potential treatment strategies.Overall, the Mouse Beta TG (Beta Thromboglobulin) ELISA Kit is a powerful tool for researchers looking to investigate the role of beta thromboglobulin in various physiological and pathological processes in mice.
Product Name:
Mouse beta-TG (beta-Thromboglobulin) ELISA Kit
SKU:
MOES01627
Target:
Mouse beta-TG (beta-Thromboglobulin)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
4.5h
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse βTG/PBP/CXCL7/NAP2. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse βTG/PBP/CXCL7/NAP2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse βTG/PBP/CXCL7/NAP2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse βTG/PBP/CXCL7/NAP2. You can calculate the concentration of Mouse βTG/PBP/CXCL7/NAP2 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
98-114
97-107
91-109
Average (%)
106
102
99
1:4
Range (%)
96-111
80-94
95-109
Average (%)
104
87
101
1:8
Range (%)
96-109
83-93
98-112
Average (%)
101
88
104
1:16
Range (%)
97-114
87-98
94-108
Average (%)
104
92
99
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
88-100
94
EDTA plasma (n=5)
84-99
90
Cell culture media (n=5)
88-100
93
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
27.6
76.9
200.7
29.0
73.8
186.4
Standard deviation
1.5
4.2
8.2
1.8
3.5
7.3
C V (%)
5.43
5.46
4.09
6.21
4.74
3.92
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Mouse βTG/PBP/CXCL7/NAP2 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse βTG/PBP/CXCL7/NAP2 in samples. No significant cross-reactivity or interference between Mouse βTG/PBP/CXCL7/NAP2 and analogues was observed.
