Mouse Beta-Site APP Cleaving Enzyme 1 (bACE1) ELISA Kit

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SKU:
MODL00142
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P56818
Sensitivity:
5.9pg/mL
Range:
15.625-1000pg/mL
ELISA Type:
Sandwich
Synonyms:
ASP2,Beta Secretase,Memapsin 2,Aspartyl protease 2,Beta-site amyloid precursor protein cleaving enzyme 1,Membrane-associated aspartic protease 2
Reactivity:
Mouse
Sample Type:
Serum, Plasma, Tissue Homogenates, Cerebrospinal Fluid And Other Biological Fluids
Frequently bought together:

Description

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Mouse Beta-Site APP Cleaving Enzyme 1 (bACE1) ELISA Kit

The Mouse BACE1 (Beta-secretase 1) ELISA Kit is a powerful tool for the precise measurement of BACE1 levels in mouse samples. This kit offers exceptional sensitivity and specificity, delivering consistent and accurate results for a variety of experimental settings.BACE1 is an important enzyme involved in the production of amyloid beta peptides, which are implicated in the pathogenesis of Alzheimer's disease. By accurately quantifying BACE1 levels, researchers can gain valuable insights into the mechanisms underlying Alzheimer's disease and potentially identify novel therapeutic targets.

The Mouse BACE1 ELISA Kit from Assay Genie is a reliable and efficient solution for studying the role of BACE1 in neurological disorders and drug development. With its user-friendly protocol and robust performance, this kit is an essential tool for researchers looking to advance their understanding of BACE1 biology.

Product Name:Mouse Beta-Site APP Cleaving Enzyme 1 (bACE1) ELISA Kit
Product Code:MODL00142
Size:96 Assays
Target:bACE1
Synonyms:ASP2, Beta Secretase, Memapsin 2, Aspartyl protease 2, Beta-site amyloid precursor protein cleaving enzyme 1, Membrane-associated aspartic protease 2
Detection Method:Sandwich
Range:15.625-1000pg/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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