Based on the information in the provided URL, the Mouse Beta EPR (Beta-Endorphin Receptor) CLIA Kit is a versatile tool for researchers looking to accurately measure beta-endorphin receptor levels in mouse serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit provides reliable and reproducible results, making it essential for a variety of research applications.The beta-endorphin receptor plays a key role in mediating the effects of beta-endorphin, a peptide involved in pain regulation, stress response, and immune function.
By accurately measuring levels of this receptor, researchers can gain valuable insights into the mechanisms underlying these processes and potentially develop targeted therapies for conditions related to pain, stress, and immune dysfunction.Overall, the Mouse Beta EPR CLIA Kit offers a powerful tool for researchers studying the beta-endorphin receptor and its role in various physiological processes, providing a valuable resource for advancing scientific understanding and therapeutic development.
Product Name:
Mouse beta-EPR (Beta-Endorphin Receptor) CLIA Kit
SKU:
MOES00117
Target:
Mouse beta-EPR (Beta-Endorphin Receptor)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
7.50 pg/mL
Detection range:
12.50-800 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse β-EPR. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse β-EPR and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse β-EPR, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse β-EPR. You can calculate the concentration of Mouse β-EPR in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
93-105
93-109
98-113
Average (%)
99
100
105
1:4
Range (%)
104-118
87-99
101-119
Average (%)
110
94
109
1:8
Range (%)
97-110
97-112
99-113
Average (%)
103
104
107
1:16
Range (%)
95-110
99-111
100-114
Average (%)
103
105
108
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
87-99
92
EDTA plasma (n=5)
101-116
107
Cell culture media (n=5)
94-110
101
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
43.63
86.07
380.31
45.15
88.37
379.39
Standard deviation
3.55
6.25
31.49
3.93
8.67
28.99
C V (%)
8.14
7.26
8.28
8.7
9.81
7.64
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse β-EPR concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse β-EPR in samples. No significant cross-reactivity or interference between Mouse β-EPR and analogues was observed.
