The Mouse BDNF (Brain-Derived Neurotrophic Factor) CLIA Kit is specially designed for the precise measurement of BDNF levels in mouse serum, plasma, and tissue extracts. This kit offers exceptional sensitivity and specificity, ensuring accurate and dependable results for a variety of research purposes.BDNF is a key neurotrophic factor that plays a vital role in regulating neuronal growth, development, and survival. Dysregulation of BDNF is associated with various neurological disorders, including Alzheimer's disease, depression, and schizophrenia, highlighting its importance as a biomarker for studying these conditions and exploring therapeutic interventions.
With its advanced technology and user-friendly design, the Mouse BDNF CLIA Kit from Assaygenie is an invaluable tool for researchers seeking to unravel the complexities of neurobiology and pave the way for innovative solutions in neuroscience and clinical medicine.
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse BDNF. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse BDNF and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse BDNF, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse BDNF. You can calculate the concentration of Mouse BDNF in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
95-111
87-103
97-112
Average (%)
103
94
103
1:4
Range (%)
90-102
94-110
94-109
Average (%)
96
101
101
1:8
Range (%)
96-109
95-110
86-100
Average (%)
103
100
93
1:16
Range (%)
100-115
96-111
94-108
Average (%)
108
103
101
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
88-100
94
EDTA plasma (n=5)
99-115
107
Cell culture media (n=5)
95-105
100
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
42.8
106.52
276.31
44.72
111.91
267.35
Standard deviation
3.94
12.27
18.43
5.65
11.47
28.42
C V (%)
9.21
11.52
6.67
12.63
10.25
10.63
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse BDNF concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse BDNF in samples. No significant cross-reactivity or interference between Mouse BDNF and analogues was observed.
