The Mouse BCL-2 (B-Cell Leukemia/Lymphoma 2) ELISA Kit is a high-quality assay designed for the precise measurement of BCL-2 levels in mouse samples, including serum, plasma, and cell culture supernatants. With its superior sensitivity and specificity, this kit delivers accurate and reproducible results, making it an excellent choice for various research applications.BCL-2 is a key regulator of apoptosis, playing a critical role in cell survival and resistance to programmed cell death.
Dysregulation of BCL-2 has been linked to various diseases, including cancer, autoimmune disorders, and neurological conditions. Thus, the Mouse BCL-2 ELISA Kit serves as a valuable tool for studying the role of BCL-2 in these diseases and exploring potential therapeutic interventions.
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse Bcl-2. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse Bcl-2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse Bcl-2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse Bcl-2. You can calculate the concentration of Mouse Bcl-2 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
92-107
85-97
97-111
Average (%)
98
92
105
1:4
Range (%)
102-119
81-95
98-112
Average (%)
108
88
105
1:8
Range (%)
96-109
86-96
97-112
Average (%)
102
91
104
1:16
Range (%)
93-107
86-97
94-108
Average (%)
101
91
99
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
85-99
91
EDTA plasma (n=5)
95-108
101
Cell culture media (n=5)
88-101
94
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
54.8
140.6
367.2
50.3
129.4
348.4
Standard deviation
3.2
6.5
18.0
3.1
7.4
18.5
C V (%)
5.84
4.62
4.9
6.16
5.72
5.31
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Mouse Bcl-2 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse Bcl-2 in samples. No significant cross-reactivity or interference between Mouse Bcl-2 and analogues was observed.
