The Mouse Bax (Bcl-2 associated X protein) ELISA Kit is specifically designed for the precise measurement of Bax levels in mouse serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit delivers accurate and reproducible results, making it an excellent choice for a variety of research applications.Bax is a key protein involved in apoptosis, playing a crucial role in regulating cell death.
Dysregulation of Bax expression has been implicated in various diseases, including cancer, neurodegenerative disorders, and cardiovascular diseases. By accurately measuring Bax levels, this ELISA kit allows researchers to study the role of Bax in these conditions and explore potential therapeutic interventions.
Product Name:
Mouse Bax (Bcl-2 Associated X Protein) ELISA Kit
SKU:
MOES00761
Target:
Mouse Bax (Bcl-2 Associated X Protein)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.10 ng/mL
Detection range:
0.16-10 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse BAX. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse BAX and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse BAX, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse BAX. You can calculate the concentration of Mouse BAX in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
88-103
86-100
90-101
Average (%)
96
93
96
1:4
Range (%)
91-109
85-100
84-96
Average (%)
99
92
90
1:8
Range (%)
87-101
84-95
90-103
Average (%)
94
89
95
1:16
Range (%)
88-102
85-95
85-99
Average (%)
94
90
92
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
86-99
91
EDTA plasma (n=5)
92-103
98
Cell culture media (n=5)
85-100
92
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.53
1.09
4.67
0.54
1.19
4.53
Standard deviation
0.04
0.06
0.19
0.03
0.05
0.17
C V (%)
7.55
5.5
4.07
5.56
4.2
3.75
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Mouse BAX concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse BAX in samples. No significant cross-reactivity or interference between Mouse BAX and analogues was observed.
