The Mouse Bax (BCL-2 Associated X Protein) CLIA Kit is designed for the precise measurement of mouse Bax levels in various biological samples, including serum, plasma, and tissue lysates. This kit offers exceptional sensitivity and specificity, ensuring accurate and reproducible results for a variety of research applications.Bax is a key protein involved in the regulation of apoptosis, playing a crucial role in cell death and survival pathways. Dysregulation of Bax expression has been linked to various diseases, including cancer and neurodegenerative disorders, highlighting its importance as a potential therapeutic target and biomarker for disease progression.
By using the Mouse Bax (BCL-2 Associated X Protein) CLIA Kit, researchers can gain valuable insights into the role of Bax in disease pathology and explore potential therapeutic interventions targeting this critical protein. Trust in the reliability and accuracy of this kit for your research needs.
Product Name:
Mouse Bax (Bcl-2 Associated X Protein) CLIA Kit
SKU:
MOES00113
Target:
Mouse Bax (Bcl-2 Associated X Protein)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse Bax. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse Bax and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse Bax, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse Bax. You can calculate the concentration of Mouse Bax in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
95-111
104-116
99-112
Average (%)
103
110
105
1:4
Range (%)
85-101
87-99
98-115
Average (%)
92
93
106
1:8
Range (%)
90-106
96-108
98-110
Average (%)
97
102
105
1:16
Range (%)
90-105
102-116
92-108
Average (%)
97
108
100
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
89-101
96
EDTA plasma (n=5)
98-114
104
Cell culture media (n=5)
97-113
104
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
104.91
281.82
931.01
94.62
264.27
885.74
Standard deviation
11.09
22.71
77.74
7.59
23.92
89.64
C V (%)
10.57
8.06
8.35
8.02
9.05
10.12
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse Bax concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse Bax in samples. No significant cross-reactivity or interference between Mouse Bax and analogues was observed.
