Mouse B-Cell CLL/Lymphoma 2 Like Protein (Bcl2L) ELISA Kit

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SKU:
MODL00148
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q64373
Sensitivity:
36.37pg/mL
Range:
78.125-5000pg/mL
ELISA Type:
Sandwich
Synonyms:
BCL-XL/S,BCL2L1,BCLX,Bcl-X,bcl-xL,bcl-xS,Apoptosis regulator Bcl-X
Reactivity:
Mouse
Sample Type:
Tissue Homogenates, Cell Lysates And Other Biological Fluids
Frequently bought together:

Description

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Mouse B-Cell CLL/Lymphoma 2 Like Protein (Bcl2L) ELISA Kit

The Mouse B-Cell CLL/Lymphoma 2-Like Protein (BCL2L) ELISA Kit is specifically designed for the quantitative measurement of BCL2L levels in mouse serum, plasma, and cell lysates. With its high sensitivity and specificity, this kit provides accurate and reliable results for a variety of research applications.BCL2L, also known as Bcl-xL, is a key regulator of apoptosis and cell survival.

Implicated in cancer, autoimmune diseases, and other pathological conditions, BCL2L serves as a valuable biomarker for studying disease progression and potential therapeutic targets. This ELISA kit offers a convenient and efficient tool for researchers seeking to investigate the role of BCL2L in various physiological and pathological processes.

Product Name:Mouse B-Cell CLL/Lymphoma 2 Like Protein (Bcl2L) ELISA Kit
Product Code:MODL00148
Size:96 Assays
Target:Bcl2L
Synonyms:BCL-XL/S, BCL2L1, BCLX, Bcl-X, bcl-xL, bcl-xS, Apoptosis regulator Bcl-X
Detection Method:Sandwich
Range:78.125-5000pg/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)83-10091
EDTA plasma (n=5)90-10396
Heparin plasma (n=5)90-9994
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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