Mouse B-Cell Activation Factor Receptor (BAFFR) ELISA Kit

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SKU:
MODL00144
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q9D8D0
Sensitivity:
1.56pg/mL
Range:
3.125-200pg/mL
ELISA Type:
Sandwich
Synonyms:
CD268,TNFRSF13C,TNFRSF13-C,BAFF-R,BAFFR,BR3,Tumor Necrosis Factor Receptor Superfamily Member 13C
Reactivity:
Mouse
Sample Type:
Serum, Plasma, Tissue Homogenates And Other Biological Fluids
Frequently bought together:

Description

system_update_altDatasheet

Mouse B-Cell Activation Factor Receptor (BAFFR) ELISA Kit

The Mouse B Cell Activation Factor Receptor (BAFFR) ELISA Kit is specifically designed for the accurate detection of BAFFR levels in mouse serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring reliable and reproducible results for various research applications.BAFFR is a critical receptor involved in regulating B cell activation and survival, playing a key role in the immune response.

Dysregulation of BAFFR signaling has been implicated in autoimmune diseases, such as systemic lupus erythematosus and rheumatoid arthritis. Therefore, the Mouse BAFFR ELISA Kit is a valuable tool for studying immune system function and potential therapeutic targets in autoimmune conditions.

Product Name:Mouse B-Cell Activation Factor Receptor (BAFFR) ELISA Kit
Product Code:MODL00144
Size:96 Assays
Target:BAFFR
Synonyms:CD268, TNFRSF13C, TNFRSF13-C, BAFF-R, BAFFR, BR3, Tumor Necrosis Factor Receptor Superfamily Member 13C
Detection Method:Sandwich
Range:3.125-200pg/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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