Mouse ATP Binding Cassette Transporter G2 (ABCG2) ELISA Kit

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SKU:
MODL00010
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q7TMS5
Sensitivity:
0.055ng/mL
Range:
0.156-10ng/mL
ELISA Type:
Sandwich
Synonyms:
CDw338,CD338,ABC-G2,MRX,BCRP1,ABC15,ABCP,BCRP,BMDP,MXR,MXR1,Mitoxantrone resistance-associated protein,Breast Cancer Resistance Protein 1,Urate exporter
Reactivity:
Mouse
Sample Type:
Tissue Homogenates And Other Biological Fluids
Frequently bought together:

Description

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Mouse ATP Binding Cassette Transporter G2 (ABCG2) ELISA Kit

The Mouse ATP Binding Cassette Transporter G2 (ABCG2) ELISA Kit is specifically designed for the accurate detection of ABCG2 levels in mouse serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring reliable and reproducible results for a variety of research applications.ABCG2 is an important protein involved in drug transport and resistance, playing a key role in protecting cells from potentially harmful substances.

Studying ABCG2 levels can provide valuable insights into drug metabolism, pharmacokinetics, and drug resistance mechanisms.With its easy-to-use format and comprehensive instructions, the Mouse ABCG2 ELISA Kit is a valuable tool for researchers studying drug transport, drug resistance, and related pathways in mouse models. Get reliable and accurate results with this high-quality ELISA kit from AssayGenie.

Product Name:Mouse ATP Binding Cassette Transporter G2 (ABCG2) ELISA Kit
Product Code:MODL00010
Size:96 Assays
Target:ABCG2
Synonyms:CDw338, CD338, ABC-G2, MRX, BCRP1, ABC15, ABCP, BCRP, BMDP, MXR, MXR1, Mitoxantrone resistance-associated protein, Breast Cancer Resistance Protein 1, Urate exporter
Detection Method:Sandwich
Range:0.156-10ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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