The Mouse AR (Amphiregulin) ELISA Kit is specifically designed for the precise measurement of amphiregulin levels in mouse serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, guaranteeing consistent and accurate results that are essential for various research purposes.Amphiregulin is a vital factor in cell growth and proliferation, playing a crucial role in processes like wound healing, tissue regeneration, and tumorigenesis.
Its involvement in signaling pathways related to cancer, inflammation, and development makes it a valuable biomarker for investigating disease mechanisms and potential therapeutic interventions.With its user-friendly protocol and high-performance capabilities, the Mouse AR ELISA Kit is an indispensable tool for researchers studying the role of amphiregulin in health and disease. Order yours today and unlock new insights into this important signaling molecule.
Product Name:
Mouse AR (Amphiregulin) ELISA Kit
SKU:
MOES01233
Target:
Mouse AR (Amphiregulin)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
4.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse AR. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse AR and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse AR, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse AR. You can calculate the concentration of Mouse AR in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
92-110
89-100
94-109
Average (%)
100
94
101
1:4
Range (%)
91-106
85-100
88-101
Average (%)
99
92
93
1:8
Range (%)
92-103
82-93
86-99
Average (%)
98
88
92
1:16
Range (%)
91-105
79-92
85-95
Average (%)
97
85
90
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
94-106
101
EDTA plasma (n=5)
94-108
101
Cell culture media (n=5)
92-105
98
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
47.7
121.26
421.86
47.42
113.62
445.04
Standard deviation
2.5
5.47
19.91
2.38
6.0
16.51
C V (%)
5.24
4.51
4.72
5.02
5.28
3.71
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Mouse AR concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse AR in samples. No significant cross-reactivity or interference between Mouse AR and analogues was observed.
