Mouse ANGPTL4 (Angiopoietin Like Protein 4) ELISA Kit
The Mouse ANGPTL4 (Angiopoietin-like Protein 4) ELISA Kit is specifically designed for the accurate quantification of ANGPTL4 levels in mouse serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and reproducible results for a variety of research applications.ANGPTL4 is a key protein involved in regulating lipid metabolism, inflammation, and angiogenesis. It plays a critical role in metabolic disorders, cardiovascular diseases, and cancer, making it a valuable biomarker for studying these conditions and exploring potential therapeutic interventions.
With its advanced design and reliable performance, the Mouse ANGPTL4 ELISA Kit is an essential tool for researchers studying the intricate mechanisms of angiogenesis and metabolic regulation in mouse models. Trust in this kit to deliver accurate and meaningful data for your research needs.
Product Name:
Mouse ANGPTL4 (Angiopoietin Like Protein 4) ELISA Kit
SKU:
MOES00695
Target:
Mouse ANGPTL4 (Angiopoietin Like Protein 4)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.10 ng/mL
Detection range:
0.16-10 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse ANGPTL4. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse ANGPTL4 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse ANGPTL4, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse ANGPTL4. You can calculate the concentration of Mouse ANGPTL4 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
92-105
93-107
86-96
Average (%)
98
99
91
1:4
Range (%)
89-99
80-92
83-96
Average (%)
94
86
88
1:8
Range (%)
86-98
80-94
82-92
Average (%)
92
86
87
1:16
Range (%)
92-106
85-97
81-96
Average (%)
99
92
88
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
86-97
92
EDTA plasma (n=5)
88-104
95
Cell culture media (n=5)
88-102
95
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.46
1.28
3.82
0.42
1.21
3.46
Standard deviation
0.03
0.05
0.18
0.02
0.05
0.14
C V (%)
6.52
3.91
4.71
4.76
4.13
4.05
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Mouse ANGPTL4 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse ANGPTL4 in samples. No significant cross-reactivity or interference between Mouse ANGPTL4 and analogues was observed.
