Mouse ANGPTL3 (Angiopoietin Like Protein 3) ELISA Kit
The Mouse ANGPTL3 (Angiopoietin-like protein 3) ELISA Kit is specifically designed for the accurate measurement of Angiopoietin-like protein 3 levels in mouse serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and reproducible results for various research applications.ANGPTL3 is a key protein involved in lipid metabolism, regulating plasma triglyceride levels and influencing lipid processing and storage. Dysregulation of ANGPTL3 has been linked to metabolic disorders such as hyperlipidemia and cardiovascular disease, making it a valuable biomarker for studying these conditions and developing potential therapeutic interventions.
By utilizing the Mouse ANGPTL3 ELISA Kit, researchers can gain valuable insights into the role of ANGPTL3 in metabolic homeostasis and its potential implications in disease progression. With its reliable performance and user-friendly protocol, this kit is an essential tool for advancing research in the field of lipid metabolism and related disorders.
Product Name:
Mouse ANGPTL3 (Angiopoietin Like Protein 3) ELISA Kit
SKU:
MOES01736
Target:
Mouse ANGPTL3 (Angiopoietin Like Protein 3)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
4.5h
Sensitivity:
0.10 ng/mL
Detection range:
0.16-10 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse ANGPTL3. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse ANGPTL3 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse ANGPTL3, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse ANGPTL3. You can calculate the concentration of Mouse ANGPTL3 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
90-106
86-98
86-97
Average (%)
97
92
92
1:4
Range (%)
90-103
82-97
89-101
Average (%)
96
89
95
1:8
Range (%)
89-102
82-94
84-97
Average (%)
96
86
91
1:16
Range (%)
92-108
80-94
88-101
Average (%)
98
86
95
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
93-105
100
EDTA plasma (n=5)
89-105
97
Cell culture media (n=5)
86-98
93
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.48
1.24
4.28
0.49
1.23
4.66
Standard deviation
0.02
0.05
0.15
0.03
0.05
0.22
C V (%)
4.17
4.03
3.5
6.12
4.07
4.72
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Mouse ANGPTL3 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse ANGPTL3 in samples. No significant cross-reactivity or interference between Mouse ANGPTL3 and analogues was observed.
