Mouse ANGPTL2 (Angiopoietin Like Protein 2) ELISA Kit
The Mouse ANGPTL2 (Angiopoietin-like Protein 2) ELISA Kit is specifically designed for the precise measurement of ANGPTL2 levels in mouse serum, plasma, and cell culture supernatants. This kit is known for its exceptional sensitivity and specificity, ensuring accurate and consistent results that are vital for a variety of research applications.ANGPTL2 is a critical protein that plays a key role in various processes such as inflammation, metabolism, and tissue remodeling.
Its dysregulation has been linked to several diseases including obesity, diabetes, and cardiovascular disorders, making it a valuable biomarker for studying these conditions and potential therapeutic interventions.Get your Mouse ANGPTL2 ELISA Kit today and unlock the potential to further explore the role of ANGPTL2 in health and disease. Visit the link below for more information:www.assaygenie.com/mouse-angptl2-angiopoietin-like-protein-2-elisa-kit-moes00694/
Product Name:
Mouse ANGPTL2 (Angiopoietin Like Protein 2) ELISA Kit
SKU:
MOES00694
Target:
Mouse ANGPTL2 (Angiopoietin Like Protein 2)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse ANGPTL2. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse ANGPTL2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse ANGPTL2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse ANGPTL2. You can calculate the concentration of Mouse ANGPTL2 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
98-111
97-114
95-110
Average (%)
105
105
102
1:4
Range (%)
94-108
88-99
86-102
Average (%)
100
93
93
1:8
Range (%)
94-106
86-99
88-100
Average (%)
100
91
95
1:16
Range (%)
92-104
86-97
85-100
Average (%)
98
91
91
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
89-104
96
EDTA plasma (n=5)
91-104
96
Cell culture media (n=5)
91-102
97
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
106.91
284.6
770.43
102.23
264.62
819.66
Standard deviation
7.13
16.31
23.42
5.58
14.13
45.0
C V (%)
6.67
5.73
3.04
5.46
5.34
5.49
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Mouse ANGPTL2 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse ANGPTL2 in samples. No significant cross-reactivity or interference between Mouse ANGPTL2 and analogues was observed.
