The Mouse ANG II (Angiotensin II) ELISA Kit is specifically designed for the accurate detection of Angiotensin II levels in mouse serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and consistent results for a variety of research applications.Angiotensin II is a key peptide hormone involved in regulating blood pressure, fluid balance, and electrolyte homeostasis. It plays a crucial role in the renin-angiotensin system, impacting various physiological processes such as vasoconstriction, inflammation, and cell growth. Abnormal Angiotensin II levels have been linked to conditions such as hypertension, heart failure, and kidney disease, making it a valuable biomarker for studying these disorders and exploring potential therapeutic interventions.
With its reliable performance and ease of use, the Mouse ANG II ELISA Kit is an essential tool for researchers investigating the role of Angiotensin II in physiological and pathological processes. Get accurate and reproducible results with this comprehensive kit, advancing your understanding of Angiotensin II biology and its implications in disease development and treatment.
Product Name:
Mouse Ang-II (Angiotensin II) ELISA Kit
SKU:
MOES01753
Target:
Mouse Ang-II (Angiotensin II)
Size:
96T
Assay type:
Competitive-ELISA
Assay time:
2.0h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Ang-Ⅱ. During the reaction, Ang-Ⅱ in the sample or standard competes with a fixed amount of Ang-Ⅱ on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Ang-Ⅱ. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Ang-Ⅱ in tested samples can be calculated by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
87-99
90-102
87-97
Average (%)
94
96
92
1:4
Range (%)
83-98
90-102
100-115
Average (%)
90
96
107
1:8
Range (%)
89-103
87-100
99-111
Average (%)
95
94
105
1:16
Range (%)
90-103
91-104
93-107
Average (%)
95
97
100
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
92-105
100
EDTA plasma (n=5)
85-101
92
Cell culture media (n=5)
86-100
91
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
46
138.0
442.8
42.5
130.8
407.7
Standard deviation
2.4
8.1
22.1
2.7
6.9
17.5
C V (%)
5.22
5.87
4.99
6.35
5.28
4.29
Sample type &Sample volume:
serum, plasma and other biological fluids; 50μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Ang-Ⅱ concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Ang-Ⅱ in samples. No significant cross-reactivity or interference between Ang-Ⅱ and analogues was observed.
