Mouse Amyloid Beta Precursor Protein Binding Protein 2 (APPBP2) ELISA Kit
The Mouse Amyloid Beta Precursor Protein Binding Protein 2 (APPBP2) ELISA Kit is a powerful tool for the accurate measurement of APPBP2 levels in mouse serum, plasma, and cell culture supernatants. With its superior sensitivity and specificity, this kit delivers precise and dependable results, making it an indispensable resource for a wide variety of research applications.APPBP2 is a key protein involved in binding to the amyloid beta precursor protein, a crucial player in the pathogenesis of Alzheimer's disease. By studying the levels of APPBP2, researchers can gain valuable insights into the mechanisms underlying this debilitating neurodegenerative disorder and potentially identify novel therapeutic targets.
This cutting-edge ELISA kit offers researchers a reliable method for quantifying APPBP2 levels, allowing for accurate measurement and comparison across samples. With its high performance and ease of use, the Mouse APPBP2 ELISA Kit is an essential tool for advancing our understanding of Alzheimer's disease and developing new treatment strategies. Visit the link to learn more: www.assaygenie.com/mouse-amyloid-beta-precursor-protein-binding-protein-2-appbp2-elisa-kit/
Product Name:
Mouse Amyloid Beta Precursor Protein Binding Protein 2 (APPBP2) ELISA Kit
Product Code:
MODL00103
Size:
96 Assays
Target:
APPBP2
Synonyms:
PAT1, Protein Interacting With APP Tail 1,Cytoplasmic Tail, Amyloid protein-binding protein 2, Protein interacting with APP tail 1
Detection Method:
Sandwich
Range:
0.312-20ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
