The Mouse AlphaFP (Alpha-fetoprotein) ELISA Kit is specifically designed for the precise measurement of alpha-fetoprotein levels in mouse serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, guaranteeing accurate and consistent results, making it perfect for various research purposes.Alpha-fetoprotein is an important biomarker associated with liver development, liver disease, and certain types of cancer.
Monitoring alpha-fetoprotein levels can provide valuable insights into these conditions, aiding in early detection and treatment strategies.Overall, the Mouse AlphaFP ELISA Kit is an essential tool for researchers studying liver health, cancer biology, and biomarker discovery in mouse models. With its reliable performance and versatile applications, this kit is a valuable asset for advancing scientific research in these areas.
Product Name:
Mouse alphaFP (Alpha-Fetoprotein) ELISA Kit
SKU:
MOES01686
Target:
Mouse alphaFP (Alpha-Fetoprotein)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.10 ng/mL
Detection range:
0.16-10 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse αFP. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse αFP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse αFP, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse αFP. You can calculate the concentration of Mouse αFP in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
90-104
84-96
95-111
Average (%)
95
91
102
1:4
Range (%)
94-108
82-94
90-103
Average (%)
100
89
97
1:8
Range (%)
96-110
85-95
96-110
Average (%)
103
90
103
1:16
Range (%)
99-117
84-96
91-104
Average (%)
107
89
98
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
89-101
96
EDTA plasma (n=5)
95-108
101
Cell culture media (n=5)
92-105
97
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.5
1.3
4.6
0.5
1.2
5.0
Standard deviation
0.03
0.07
0.16
0.03
0.07
0.23
C V (%)
6
5.38
3.48
6.0
5.83
4.6
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Mouse αFP concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse αFP in samples. No significant cross-reactivity or interference between Mouse αFP and analogues was observed.
