The Mouse Alpha1-AGP (Alpha1 Acid Glycoprotein) CLIA Kit is a powerful tool for accurately measuring levels of Alpha1-AGP in mouse serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit delivers reliable and consistent results for a wide range of research applications.Alpha1-AGP is a key glycoprotein involved in acute phase responses, inflammation, and drug binding in mammals.
By studying Alpha1-AGP levels, researchers can gain valuable insights into various disease states, including cancer, infectious diseases, and inflammatory conditions. This kit is essential for exploring the role of Alpha1-AGP in disease progression and for developing targeted therapeutic strategies.
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse AGP1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse AGP1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse AGP1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse AGP1. You can calculate the concentration of Mouse AGP1 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
89-102
90-103
99-115
Average (%)
96
97
107
1:4
Range (%)
104-115
89-105
100-115
Average (%)
109
97
108
1:8
Range (%)
100-116
100-116
88-100
Average (%)
108
107
93
1:16
Range (%)
86-99
88-100
101-117
Average (%)
93
95
107
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
97-111
105
EDTA plasma (n=5)
89-102
94
Cell culture media (n=5)
101-114
107
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
4.0
9.01
37.17
4.27
9.4
37.33
Standard deviation
0.35
0.8
2.69
0.38
0.73
3.34
C V (%)
8.75
8.88
7.24
8.9
7.77
8.95
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse AGP1 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse AGP1 in samples. No significant cross-reactivity or interference between Mouse AGP1 and analogues was observed.
