The Mouse Alpha-Endorphin (AEP) ELISA Kit from Assay Genie is a powerful tool for the precise measurement of levels of alpha-endorphin in mouse serum, plasma, and tissue culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and consistent results for a diverse range of research applications.Alpha-endorphin is a neuropeptide with potent analgesic and anti-inflammatory properties, playing a crucial role in pain modulation and stress response. It is involved in various physiological processes and has been implicated in conditions such as chronic pain, mood disorders, and addiction.
Monitoring alpha-endorphin levels can provide valuable insights into these conditions and aid in the development of novel therapeutic strategies.Researchers can rely on the Mouse Alpha-Endorphin (AEP) ELISA Kit to deliver reliable and reproducible data, facilitating in-depth investigations into the roles of alpha-endorphin in health and disease. With its user-friendly protocol and high-performance characteristics, this kit is an indispensable resource for advancing scientific knowledge and driving innovative discoveries in the field of neurobiology.
Product Name:
Mouse Alpha-Endorphin (aEP) ELISA Kit
Product Code:
MODL00036
Size:
96 Assays
Target:
aEP
Detection Method:
Competition
Range:
15.6-1,000pg/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-99
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. Note:To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity, and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 50µL standard or sample to each well. And then add 50µL prepared Detection Reagent A immediately. Shake and mix. Incubate 1 hour at 37°C
3.
Aspirate and wash 3 times
4.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
5.
Aspirate and wash 5 times
6.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
7.
Add 50µL Stop Solution. Read at 450 nm immediately.
