For our Mouse AGRP (Agouti-Related Protein) ELISA Kit, it is specifically designed for the accurate quantification of AGRP levels in mouse serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring reliable and reproducible results for various research applications.AGRP is a key protein involved in regulating energy balance and feeding behavior in mice.
It plays a critical role in the development of obesity and metabolic disorders, making it a valuable biomarker for studying these conditions and exploring potential treatments.Overall, our Mouse AGRP ELISA Kit provides researchers with a powerful tool to delve deeper into the role of AGRP in physiological processes and disease pathology in mice.
Product Name:
Mouse AgRP (Agouti Related Protein) ELISA Kit
SKU:
MOES01668
Target:
Mouse AgRP (Agouti Related Protein)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse AgRP. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse AgRP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse AgRP, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse AgRP. You can calculate the concentration of Mouse AgRP in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
98-112
95-109
88-100
Average (%)
106
102
94
1:4
Range (%)
87-98
81-91
84-97
Average (%)
93
86
89
1:8
Range (%)
91-104
88-99
85-99
Average (%)
97
93
92
1:16
Range (%)
85-100
85-97
81-94
Average (%)
92
89
88
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
91-107
98
EDTA plasma (n=5)
94-110
100
Cell culture media (n=5)
88-101
94
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
49.42
133.44
451.5
49.92
122.91
452.7
Standard deviation
2.63
5.42
21.31
2.92
5.65
20.46
C V (%)
5.32
4.06
4.72
5.85
4.6
4.52
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Mouse AgRP concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse AgRP in samples. No significant cross-reactivity or interference between Mouse AgRP and analogues was observed.
