Receptor for Advanced Glycosylation End Products (RAGE, or AGER) is a member of the immunoglobulin super-family transmembrane proteins, as a signal transduction receptor which binds advanced glycation endproducts, certain members of the S100/calgranulin family of proteins, high mobility group box 1 (HMGB1), advanced oxidation protein products, and amyloid (beta-sheet fibrils). Initial studies investigating the role of RAGE in renal dysfunction focused on diabetes, neurodegenerative disorders, and inflammatory responses. However, RAGE also has roles in the pathogenesis of renal disorders that are not associated with diabetes, such as obesity-related glomerulopathy, doxorubicin-induced nephropathy, hypertensive nephropathy, lupus nephritis, renal amyloidosis, and ischemic renal injuries. RAGE represents an important factor in innate immunity against pathogens, but it also interacts with endogenous ligands, resulting in chronic inflammation. RAGE signaling has been implicated in multiple human illnesses, including atherosclerosis, arthritis, Alzheimer's disease, atherosclerosis and aging associated diseases.
Product Name:
Mouse AGER/RAGE Recombinant Protein (RPES2505)
Product Code:
RPES2505
Size:
50µg
Species:
Mouse
Expressed Host:
HEK293 Cells
Synonyms:
RAGE
Accession:
NP_031451.2
Sequence:
Met 1-Ala 342
Fusion tag:
C-His
Activity:
Measured by its ability to bind mouse HMGB1-Fc in functional ELISA.
Endotoxin:
<1.0 EU per µg of the protein as determined by the LAL method.
Protein Construction:
A DNA sequence encoding the extracellular domain of mouse AGER (NP_031451.2) extracellular domain (Met 1-Ala 342) was expressed, with a polyhistidine tag at the C-terminus.
Purity:
> 96 % as determined by SDS-PAGE
Mol Mass:
35.3 kDa
AP Mol Mass:
48 kDa
Formulation:
Lyophilized from sterile PBS, pH 7.4
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
