The Mouse ADP (Adiponectin) ELISA Kit is specifically designed for the quantitative detection of mouse adiponectin levels in serum, plasma, and cell culture supernatants. This kit provides high sensitivity and specificity, ensuring accurate and reliable results for a variety of research applications.Adiponectin is a key adipokine that plays a crucial role in regulating glucose and lipid metabolism, as well as inflammation and insulin sensitivity.
It is an important biomarker for metabolic diseases such as obesity, type 2 diabetes, and cardiovascular disease, making it essential for studying these conditions and potential therapeutic interventions.Overall, the Mouse ADP (Adiponectin) ELISA Kit is a valuable tool for researchers looking to explore the role of adiponectin in various physiological and pathological processes in mouse models.
Product Name:
Mouse ADP/Acrp30 (Adiponectin) ELISA Kit
SKU:
MOES00623
Target:
Mouse ADP/Acrp30 (Adiponectin)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse ADP/Acrp30. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse ADP/Acrp30 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse ADP/Acrp30, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse ADP/Acrp30. You can calculate the concentration of Mouse ADP/Acrp30 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
99-112
92-106
85-96
Average (%)
106
100
91
1:4
Range (%)
100-112
92-106
87-100
Average (%)
106
100
93
1:8
Range (%)
99-114
92-108
81-95
Average (%)
107
100
87
1:16
Range (%)
94-110
93-106
88-98
Average (%)
102
100
93
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
90-100
95
EDTA plasma (n=5)
93-111
101
Cell culture media (n=5)
86-99
93
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
49.92
135.68
423.36
53.1
140.06
384.77
Standard deviation
2.56
7.18
19.86
3.61
7.26
20.78
C V (%)
5.13
5.29
4.69
6.8
5.18
5.4
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Mouse ADP/Acrp30 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse ADP/Acrp30 in samples. No significant cross-reactivity or interference between Mouse ADP/Acrp30 and analogues was observed.
