Anti-Mono-methyl-HIST1H1C (K105) 항체의 (PACO56676)

Antibody Name:	Mono-methyl-HIST1H1C (K105) Antibody (PACO56676)
Antibody SKU:	PACO56676
Size:	50ul
Host Species:	Rabbit
Tested Applications:	ELISA, IF
Recommended Dilutions:	ELISA:1:2000-1:10000, IF:1:1-1:10
Species Reactivity:	Human
Immunogen:	Peptide sequence around site of Mono-methyl-Lys (105) derived from Human Histone H1.2

Form:	Liquid
Storage Buffer:	Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Purification Method:	Antigen Affinity Purified
Clonality:	Polyclonal
Isotype:	IgG
Conjugate:	Non-conjugated

Immunofluorescence staining of Hela cells with PACO56676 at 1:2.5, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Background:	Histone H1 protein binds to linker DNA between nucleosomes forming the macromolecular structure known as the chromatin fiber. Histones H1 are necessary for the condensation of nucleosome chains into higher-order structured fibers. Acts also as a regulator of individual gene transcription through chromatin remodeling, nucleosome spacing and DNA methylation (By similarity).
Synonyms:	Histone H1.2 (Histone H1c) (Histone H1d) (Histone H1s-1), HIST1H1C, H1F2

UniProt Protein Function:	H1C: Histone H1 protein binds to linker DNA between nucleosomes forming the macromolecular structure known as the chromatin fiber. Histones H1 are necessary for the condensation of nucleosome chains into higher-order structured fibers. Acts also as a regulator of individual gene transcription through chromatin remodeling, nucleosome spacing and DNA methylation. Belongs to the histone H1/H5 family.
UniProt Protein Details:	Protein type:DNA-binding Chromosomal Location of Human Ortholog: 6p21.3 Cellular Component: nucleus Molecular Function:chromatin DNA binding; protein binding
NCBI Summary:	Histones are basic nuclear proteins responsible for nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H1 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6. [provided by RefSeq, Aug 2015]
UniProt Code:	P16403
NCBI GenInfo Identifier:	417101
NCBI Gene ID:	3006
NCBI Accession:	P16403.2
UniProt Secondary Accession:	P16403,A8K4I2,
UniProt Related Accession:	P16403
Molecular Weight:	21,365 Da
NCBI Full Name:	Histone H1.2
NCBI Synonym Full Names:	histone cluster 1, H1c
NCBI Official Symbol:	HIST1H1C
NCBI Official Synonym Symbols:	H1C; H1.2; H1F2; H1s-1
NCBI Protein Information:	histone H1.2
UniProt Protein Name:	Histone H1.2
UniProt Synonym Protein Names:	Histone H1c; Histone H1d; Histone H1s-1
UniProt Gene Name:	HIST1H1C
UniProt Entry Name:	H12_HUMAN

Antibodies

HIST1H1C Antibody (PACO09730)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)