The Monkey TARC (Thymus Activation Regulated Chemokine) ELISA Kit is a powerful tool for the accurate measurement of TARC levels in monkey serum, plasma, and cell culture supernatants. With its superior sensitivity and specificity, this kit provides reliable and consistent results, making it an indispensable tool for a variety of research applications.TARC is a key chemokine involved in immune response regulation, specifically in the recruitment of T lymphocytes to sites of inflammation.
Dysregulation of TARC expression has been associated with various inflammatory diseases, making it a valuable biomarker for studying these conditions and developing potential therapeutic strategies.With the Monkey TARC ELISA Kit, researchers can gain valuable insights into the role of TARC in immune function and inflammatory diseases, paving the way for new advancements in research and drug development.
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Monkey TARC. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Monkey TARC and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Monkey TARC, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Monkey TARC. You can calculate the concentration of Monkey TARC in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
90-103
85-100
91-105
Average (%)
98
91
98
1:4
Range (%)
93-107
82-96
88-103
Average (%)
99
89
94
1:8
Range (%)
93-109
87-98
89-101
Average (%)
100
93
94
1:16
Range (%)
91-102
84-94
91-105
Average (%)
96
89
97
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
89-103
95
EDTA plasma (n=5)
86-100
92
Cell culture media (n=5)
86-97
92
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
103.72
268.13
859.21
96.66
286.22
824.6
Standard deviation
6.59
11.18
30.85
6.53
11.56
27.54
C V (%)
6.35
4.17
3.59
6.76
4.04
3.34
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Monkey TARC concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Monkey TARC in samples. No significant cross-reactivity or interference between Monkey TARC and analogues was observed.
