The Monkey OC/BGP (Osteocalcin/Bone Gla Protein) ELISA Kit is specifically designed for the accurate measurement of osteocalcin and bone gla protein levels in monkey serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and reproducible results for a variety of research applications.Osteocalcin and bone gla protein are key markers for bone metabolism and health, playing essential roles in bone formation and turnover. Abnormal levels of these proteins can indicate bone-related disorders such as osteoporosis, osteopenia, and bone fractures.
By accurately measuring these biomarkers, researchers can gain valuable insights into bone health and diseases, ultimately leading to the development of potential therapies and treatments.The Monkey OC/BGP ELISA Kit from Assay Genie is a valuable tool for researchers studying bone biology, musculoskeletal disorders, and bone-related diseases in monkeys. Its user-friendly design and high-performance features make it a reliable choice for quantitative analysis of osteocalcin and bone gla protein levels in various biological samples.
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Monkey OC/BGP. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Monkey OC/BGP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Monkey OC/BGP, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Monkey OC/BGP. You can calculate the concentration of Monkey OC/BGP in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
86-100
86-96
95-109
Average (%)
91
91
101
1:4
Range (%)
93-107
80-89
89-101
Average (%)
99
85
94
1:8
Range (%)
93-104
82-96
87-100
Average (%)
98
87
93
1:16
Range (%)
92-108
83-98
86-101
Average (%)
99
90
92
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
95-106
100
EDTA plasma (n=5)
97-112
102
Cell culture media (n=5)
86-100
92
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
2.7
6.48
21.99
2.85
7.09
22.11
Standard deviation
0.14
0.3
0.93
0.15
0.34
0.82
C V (%)
5.19
4.63
4.23
5.26
4.8
3.71
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Monkey OC/BGP concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Monkey OC/BGP in samples. No significant cross-reactivity or interference between Monkey OC/BGP and analogues was observed.
