The Monkey LCN2 (Lipocalin 2) ELISA Kit is specifically designed for the accurate detection of lipocalin 2 levels in monkey serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and consistent results for use in a variety of research applications.Lipocalin 2 is a key protein involved in various physiological processes, including immune response and iron transport.
Dysregulation of lipocalin 2 has been implicated in a range of diseases, such as inflammation, kidney disease, and cancer, making it a valuable biomarker for studying these conditions and developing potential therapeutic interventions.Overall, the Monkey LCN2 (Lipocalin 2) ELISA Kit provides researchers with a reliable tool for investigating the role of lipocalin 2 in monkey biology and disease pathogenesis.
Product Name:
Monkey LCN2 (Lipocalin 2) ELISA Kit (MKES00040)
SKU:
MKES00040
Target:
Monkey LCN2 (Lipocalin 2)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Monkey LCN2. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Monkey LCN2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Monkey LCN2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Monkey LCN2. You can calculate the concentration of Monkey LCN2 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
96-109
95-112
90-104
Average (%)
102
102
95
1:4
Range (%)
91-105
99-112
89-105
Average (%)
97
105
96
1:8
Range (%)
97-112
90-104
85-100
Average (%)
104
97
92
1:16
Range (%)
97-109
92-107
92-106
Average (%)
103
97
97
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
93-109
100
EDTA plasma (n=5)
92-110
100
Cell culture media (n=5)
89-106
97
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
1.01
2.8
6.83
0.93
2.95
6.42
Standard deviation
0.06
0.16
0.34
0.06
0.15
0.35
C V (%)
5.94
5.71
4.98
6.45
5.08
5.43
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Monkey LCN2 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Monkey LCN2 in samples. No significant cross-reactivity or interference between Monkey LCN2 and analogues was observed.
