Monkey Interleukin 1 Receptor Antagonist (IL-1RA) ELISA Kit

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SKU:
MKDL00020
Product Type:
ELISA Kit
Sensitivity:
5.8pg/mL
Range:
15.625-1000pg/mL
ELISA Type:
Sandwich
Synonyms:
IL1RN,IL1-RA,ICIL1RA,IL1ra3,IL1F3,IRAP
Reactivity:
Monkey
Sample Type:
Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernatants And Other Biological Fluids
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Description

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Monkey Interleukin 1 Receptor Antagonist (IL-1RA) ELISA Kit

The Monkey Interleukin-1 Receptor Antagonist (IL1RA) ELISA Kit is designed for precise measurement of IL1RA levels in monkey serum, plasma, and cell culture supernatants. This kit is known for its high sensitivity and specificity, ensuring accurate and reproducible results for various research applications.IL1RA is a key regulator of the immune response, inhibiting the activity of interleukin-1 and reducing inflammation.

Dysregulation of IL1RA has been linked to various inflammatory conditions, autoimmune diseases, and neurological disorders, making it a valuable biomarker for studying these diseases and developing potential treatments.Overall, the Monkey IL1RA ELISA Kit provides researchers with a reliable tool to investigate the role of IL1RA in disease pathogenesis and explore potential therapeutic interventions.

Product Name:Monkey Interleukin 1 Receptor Antagonist (IL-1RA) ELISA Kit
Product Code:MKDL00020
Size:96 Assays
Target:IL1RA
Synonyms:IL1RN, IL1-RA, ICIL1RA, IL1ra3, IL1F3, IRAP, IL-1RA, Interleukin-1 Family Member 3, IL1 inhibitor, Anakinra
Detection Method:Sandwich
Range:15.625-1000pg/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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