The Monkey GZMB (Granzyme B) ELISA Kit is specifically designed for the accurate measurement of Granzyme B levels in monkey samples including serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, researchers can depend on reliable and reproducible results for various research applications.Granzyme B is an important protease enzyme found in cytotoxic T cells and natural killer cells, playing a key role in immune response and apoptosis.
Elevated levels of Granzyme B have been linked to various inflammatory and autoimmune diseases, making it a valuable biomarker for studying these conditions and potential therapeutic interventions.Overall, the Monkey GZMB (Granzyme B) ELISA Kit offers researchers a valuable tool for studying immune responses and disease mechanisms in monkeys, providing crucial insights for advancing research in immunology and related fields.
Product Name:
Monkey GzmB (Granzyme B) ELISA Kit (MKES00030)
SKU:
MKES00030
Target:
Monkey GzmB (Granzyme B)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Monkey GzmB. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Monkey GzmB and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Monkey GzmB, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Monkey GzmB. You can calculate the concentration of Monkey GzmB in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
94-107
90-103
92-105
Average (%)
100
97
99
1:4
Range (%)
94-107
93-105
90-106
Average (%)
99
99
97
1:8
Range (%)
93-109
91-107
92-105
Average (%)
99
97
97
1:16
Range (%)
96-112
90-104
93-105
Average (%)
103
98
99
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
90-104
96
EDTA plasma (n=5)
91-105
96
Cell culture media (n=5)
83-99
90
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
48.48
114.27
351.76
44.88
103.03
327.76
Standard deviation
3.24
6.46
11.08
2.57
4.43
16.36
C V (%)
6.68
5.65
3.15
5.73
4.3
4.99
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Monkey GzmB concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Monkey GzmB in samples. No significant cross-reactivity or interference between Monkey GzmB and analogues was observed.
