The Monkey GH (Growth Hormone) ELISA Kit is a specialized assay designed for the precise measurement of growth hormone levels in monkey serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit delivers accurate and reproducible results, making it an invaluable tool for researchers in various fields.Growth hormone plays a pivotal role in regulating growth, metabolism, and overall body composition. Abnormal levels of growth hormone have been associated with various disorders and conditions, making it a critical biomarker for studying growth-related diseases and potential therapeutic interventions.
This ELISA kit from AssayGenie provides researchers with a reliable and efficient method for quantifying growth hormone levels in monkey samples, enabling in-depth investigations and advancements in the field of endocrinology and growth research.
Product Name:
Monkey GH (Growth Hormone) ELISA Kit (MKES00001)
SKU:
MKES00001
Target:
Monkey GH (Growth Hormone)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.47 ng/mL
Detection range:
0.78-50 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Monkey GH. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Monkey GH and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Monkey GH, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Monkey GH. You can calculate the concentration of Monkey GH in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
93-110
89-102
86-101
Average (%)
100
95
92
1:4
Range (%)
88-102
79-95
83-94
Average (%)
95
86
89
1:8
Range (%)
86-101
81-93
87-97
Average (%)
92
86
92
1:16
Range (%)
87-101
83-94
85-96
Average (%)
93
88
90
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
93-109
100
EDTA plasma (n=5)
91-103
96
Cell culture media (n=5)
91-106
97
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
2.63
7.38
22.45
2.69
7.28
24.21
Standard deviation
0.17
0.34
0.86
0.17
0.33
0.95
C V (%)
6.46
4.61
3.83
6.32
4.53
3.92
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Monkey GH concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Monkey GH in samples. No significant cross-reactivity or interference between Monkey GH and analogues was observed.
