The Monkey CG Beta (Chorionic Gonadotrophin Beta) ELISA Kit is a reliable and accurate tool for detecting levels of CG Beta in monkey serum, plasma, and cell culture supernatants. This kit boasts high sensitivity and specificity, ensuring precise and consistent results for a variety of research applications.CG Beta is a key hormone involved in reproductive health, specifically in the early stages of pregnancy. Monitoring CG Beta levels can provide valuable insights into fertility and pregnancy-related disorders in monkeys, making this ELISA kit essential for researchers studying reproductive biology and potential therapeutic interventions.
Overall, the Monkey CG Beta ELISA Kit offers researchers a valuable tool for investigating reproductive health in monkeys, with the potential to advance our understanding of fertility and pregnancy-related conditions in these animals.
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Monkey CGβ. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Monkey CGβ and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Monkey CGβ, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Monkey CGβ. You can calculate the concentration of Monkey CGβ in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
94-108
85-100
94-109
Average (%)
101
91
101
1:4
Range (%)
86-101
84-99
82-95
Average (%)
93
90
89
1:8
Range (%)
92-105
81-94
87-101
Average (%)
98
87
92
1:16
Range (%)
95-105
81-94
85-98
Average (%)
100
86
91
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
92-109
99
EDTA plasma (n=5)
93-104
98
Cell culture media (n=5)
87-99
93
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
2.33
7.94
21.13
2.4
8.0
20.85
Standard deviation
0.16
0.34
1.06
0.13
0.38
0.79
C V (%)
6.87
4.28
5.02
5.42
4.75
3.79
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Monkey CGβ concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Monkey CGβ in samples. No significant cross-reactivity or interference between Monkey CGβ and analogues was observed.
