Monkey CD163 (Cluster of Differentiation 163) ELISA Kit (MKES00023)
The Monkey CD163 (Cluster of Differentiation 163) ELISA Kit is specifically designed for the quantitative detection of CD163 levels in monkey serum, plasma, and tissue homogenates. This kit offers high sensitivity and specificity, allowing for precise and accurate results that are essential for research studies in various disciplines.CD163 is a key cell surface marker that plays a critical role in the regulation of inflammation and immune responses. It is particularly important in the context of inflammatory diseases, autoimmune disorders, and infectious conditions.
The Monkey CD163 ELISA Kit provides researchers with a valuable tool for investigating the role of CD163 in disease pathogenesis and identifying potential therapeutic targets.Overall, the Monkey CD163 ELISA Kit from Assay Genie is a reliable and efficient assay system that is tailored to meet the specific needs of researchers working with monkey models. By providing robust and reproducible data, this kit is an indispensable resource for advancing our understanding of CD163 biology and its implications for human health.
Product Name:
Monkey CD163 (Cluster of Differentiation 163) ELISA Kit (MKES00023)
SKU:
MKES00023
Target:
Monkey CD163 (Cluster of Differentiation 163)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.94 ng/mL
Detection range:
1.56-100 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Monkey CD163. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Monkey CD163 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Monkey CD163, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Monkey CD163. You can calculate the concentration of Monkey CD163 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
87-102
94-105
97-108
Average (%)
94
99
102
1:4
Range (%)
83-95
90-105
94-108
Average (%)
90
97
100
1:8
Range (%)
84-96
91-106
93-106
Average (%)
89
97
99
1:16
Range (%)
89-102
96-110
97-109
Average (%)
95
101
102
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
91-108
99
EDTA plasma (n=5)
87-102
94
Cell culture media (n=5)
87-102
94
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
4.7
8.37
49.5
4.53
8.3
51.13
Standard deviation
0.27
0.42
2.47
0.24
0.4
2.75
C V (%)
5.74
5.02
4.99
5.3
4.82
5.37
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Monkey CD163 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Monkey CD163 in samples. No significant cross-reactivity or interference between Monkey CD163 and analogues was observed.
