The Monkey C5a (Complement Component 5a) ELISA Kit is a powerful tool for accurate measurement of C5a levels in monkey serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit delivers reliable and reproducible results, making it perfectly suited for a variety of research applications.C5a is a key component of the complement system, playing a crucial role in inflammation, immune response, and host defense. Dysregulation of C5a has been implicated in various diseases, including sepsis, autoimmune disorders, and inflammatory conditions.
By accurately measuring C5a levels, researchers can gain valuable insights into the mechanisms underlying these pathological processes and explore new therapeutic targets.With the Monkey C5a ELISA Kit, researchers can confidently study the role of C5a in monkey models, furthering our understanding of immune regulation and inflammatory diseases. Don't miss out on this essential tool for your research endeavors.
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Monkey C5a. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Monkey C5a and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Monkey C5a, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Monkey C5a. You can calculate the concentration of Monkey C5a in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
91-108
92-108
84-97
Average (%)
99
99
91
1:4
Range (%)
95-108
90-105
82-92
Average (%)
100
97
87
1:8
Range (%)
93-109
93-106
81-95
Average (%)
100
100
88
1:16
Range (%)
93-106
95-109
88-101
Average (%)
100
100
93
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
86-101
92
EDTA plasma (n=5)
93-107
99
Cell culture media (n=5)
86-101
92
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.53
1.02
3.8
0.57
1.07
3.97
Standard deviation
0.03
0.05
0.12
0.03
0.06
0.22
C V (%)
5.66
4.9
3.16
5.26
5.61
5.45
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Monkey C5a concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Monkey C5a in samples. No significant cross-reactivity or interference between Monkey C5a and analogues was observed.
