Monkey ANGPTL3 (Angiopoietin Like Protein 3) ELISA Kit (MKES00016)
The Monkey ANGPTL3 (Angiopoietin-like Protein 3) ELISA Kit is specifically designed for the precise measurement of ANGPTL3 levels in monkey serum, plasma, and cell culture supernatants. This ELISA kit is known for its superior sensitivity and specificity, guaranteeing accurate and consistent results for various research purposes.ANGPTL3 is a vital protein involved in lipid metabolism and regulation, influencing factors such as triglyceride levels and lipid storage.
Dysregulation of ANGPTL3 has been linked to metabolic disorders such as dyslipidemia and cardiovascular diseases, making it a valuable biomarker for studying these conditions and exploring potential therapeutic interventions.With the Monkey ANGPTL3 ELISA Kit, researchers can confidently study the role of ANGPTL3 in monkey models, advancing our understanding of lipid metabolism and its implications for human health.
Product Name:
Monkey ANGPTL3 (Angiopoietin Like Protein 3) ELISA Kit (MKES00016)
SKU:
MKES00016
Target:
Monkey ANGPTL3 (Angiopoietin Like Protein 3)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
23.44 pg/mL
Detection range:
39.06-2500 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Monkey ANGPTL3. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Monkey ANGPTL3 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Monkey ANGPTL3, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Monkey ANGPTL3. You can calculate the concentration of Monkey ANGPTL3 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
94-109
97-108
85-101
Average (%)
100
103
92
1:4
Range (%)
90-101
99-110
83-93
Average (%)
96
105
88
1:8
Range (%)
96-108
97-114
88-103
Average (%)
102
104
95
1:16
Range (%)
95-110
92-106
82-96
Average (%)
102
98
88
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
83-95
90
EDTA plasma (n=5)
92-106
99
Cell culture media (n=5)
84-98
91
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
122.04
257.18
1106.61
130.53
240.66
1023.53
Standard deviation
7.93
13.73
40.06
8.3
12.13
39.51
C V (%)
6.5
5.34
3.62
6.36
5.04
3.86
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Monkey ANGPTL3 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Monkey ANGPTL3 in samples. No significant cross-reactivity or interference between Monkey ANGPTL3 and analogues was observed.
