Map2k4 Antibody (PACO28138)
- SKU:
- PACO28138
- Product type:
- Antibody
- Reactivity:
- Mouse
- Rat
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Application:
- ELISA
- Application:
- WB
- Antibody type:
- Polyclonal
- Conjugation:
- Unconjugated
Frequently bought together:
Description
| Antibody Name: | Map2k4 Antibody (PACO28138) |
| Antibody SKU: | PACO28138 |
| Size: | 50ug |
| Host Species: | Rabbit |
| Tested Applications: | ELISA, WB |
| Recommended Dilutions: | ELISA:1:2000-1:10000, WB:1:500-1:5000 |
| Species Reactivity: | Mouse, Rat, Human |
| Immunogen: | Recombinant Mouse Dual specificity mitogen-activated protein kinase kinase 4 protein (2-397AA) |
| Form: | Liquid |
| Storage Buffer: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
| Purification Method: | >95%, Protein G purified |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Conjugate: | Non-conjugated |
 | Western Blot Positive WB detected in: RAW264.7 whole cell lysate, HepG2 whole cell lysate, Rat liver tissue, Mouse brain tissue All lanes: Map2k4 antibody at 3.2µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 45 kDa Observed band size: 45 kDa . |
| Background: | Dual specificity protein kinase which acts as an essential component of the MAP kinase signal transduction pathway. Essential component of the stress-activated protein kinase/c-Jun N-terminal kinase (SAP/JNK) signaling pathway. With MAP2K7/MKK7, is the one of the only known kinase to directly activate the stress-activated protein kinase/c-Jun N-terminal kinases MAPK8/JNK1, MAPK9/JNK2 and MAPK10/JNK3. MAP2K4/MKK4 and MAP2K7/MKK7 both activate the JNKs by phosphorylation, but they differ in their preference for the phosphorylation site in the Thr-Pro-Tyr motif. MAP2K4 shows preference for phosphorylation of the Tyr residue and MAP2K7/MKK7 for the Thr residue. The phosphorylation of the Thr residue by MAP2K7/MKK7 seems to be the prerequisite for JNK activation at least in response to proinflammatory cytokines, while other stimuli activate both MAP2K4/MKK4 and MAP2K7/MKK7 which synergistically phosphorylate JNKs. MAP2K4 is required for maintaining peripheral lymphoid homeostasis. The MKK/JNK signaling pathway is also involved in mitochondrial death signaling pathway, including the release cytochrome c, leading to apoptosis. Whereas MAP2K7/MKK7 exclusively activates JNKs, MAP2K4/MKK4 additionally activates the p38 MAPKs MAPK11, MAPK12, MAPK13 and MAPK14. |
| Synonyms: | Dual specificity mitogen-activated protein kinase kinase 4 (MAP kinase kinase 4) (MAPKK 4) (EC 2.7.12.2) (C-JUN N-terminal kinase kinase 1) (JNK kinase 1) (JNKK 1) (JNK-activating kinase 1) (MAPK/ERK kinase 4) (MEK 4) (SAPK/ERK kinase 1) (SEK1), Map2k4, Jnkk1 Mek4 Mkk4 Prkmk4 Sek1 Serk1 Skk1 |
| UniProt Protein Function: | MKK4: dual specificity kinase of the STE7 family that phosphorylates and activates JNK1 and -2 as well as p38 but not ERK1 or -2. Mediates cellular responses to various cellular stresses and inflammatory cytokines. Phosphorylation by Akt inhibits MKK4 and suppresses stress-activated signal transduction.Protein type: Protein kinase, dual-specificity (non-receptor); Protein kinase, STE; EC 2.7.12.2; Kinase, protein; STE group; STE7 familyCellular Component: axon; cytoplasm; cytosol; dendrite cytoplasm; nucleus; perikaryonMolecular Function: ATP binding; JUN kinase kinase activity; MAP kinase kinase activity; mitogen-activated protein kinase kinase kinase binding; protein binding; receptor signaling protein serine/threonine kinase activityBiological Process: activation of JNK activity; JNK cascade; MAPKKK cascade; positive regulation of apoptosis; positive regulation of DNA replication; positive regulation of neuron apoptosis; positive regulation of nitric-oxide synthase biosynthetic process; positive regulation of protein amino acid phosphorylation; protein amino acid phosphorylation; response to wounding |
| UniProt Protein Details: | |
| NCBI Summary: | |
| UniProt Code: | P47809 |
| NCBI GenInfo Identifier: | 2507202 |
| NCBI Gene ID: | 26398 |
| NCBI Accession: | P47809.2 |
| UniProt Secondary Accession: | P47809 |
| UniProt Related Accession: | P47809 |
| Molecular Weight: | 44,114 Da |
| NCBI Full Name: | Dual specificity mitogen-activated protein kinase kinase 4 |
| NCBI Synonym Full Names: | mitogen-activated protein kinase kinase 4 |
| NCBI Official Symbol: | Map2k4 |
| NCBI Official Synonym Symbols: | MEK4; MKK4; Sek1; JNKK1; Serk1; PRKMK4 |
| NCBI Protein Information: | dual specificity mitogen-activated protein kinase kinase 4 |
| UniProt Protein Name: | Dual specificity mitogen-activated protein kinase kinase 4 |
| UniProt Synonym Protein Names: | C-JUN N-terminal kinase kinase 1; JNK kinase 1; JNKK 1; JNK-activating kinase 1; MAPK/ERK kinase 4; MEK 4; SAPK/ERK kinase 1; SEK1 |
| Protein Family: | Dual specificity mitogen-activated protein kinase kinase |
| UniProt Gene Name: | Map2k4 |
| UniProt Entry Name: | MP2K4_MOUSE |
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