MACC1 Antibody (PACO55982)
- SKU:
- PACO55982
- Product type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Application:
- ELISA
- Application:
- WB
- Application:
- IHC
- Application:
- IF
- Antibody type:
- Polyclonal
- Conjugation:
- Unconjugated
Frequently bought together:
Description
Antibody Name: | MACC1 Antibody (PACO55982) |
Antibody SKU: | PACO55982 |
Size: | 50ug |
Host Species: | Rabbit |
Tested Applications: | ELISA, WB, IHC, IF |
Recommended Dilutions: | ELISA:1:2000-1:10000, WB:1:500-1:5000, IHC:1:20-1:200, IF:1:50-1:200 |
Species Reactivity: | Human |
Immunogen: | Recombinant Human Metastasis-associated in colon cancer protein 1 protein (371-514AA) |
Form: | Liquid |
Storage Buffer: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
Purification Method: | >95%, Protein G purified |
Clonality: | Polyclonal |
Isotype: | IgG |
Conjugate: | Non-conjugated |
Western Blot. Positive WB detected in: Hela whole cell lysate, 293 whole cell lysate, A549 whole cell lysate, HepG2 whole cell lysate. All lanes: MACC1 antibody at 3µg/ml. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 97 kDa. Observed band size: 97 kDa. | |
Immunofluorescence staining of MCF-7 cells with PACO55982 at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). | |
IHC image of PACO55982 diluted at 1:100 and staining in paraffin-embedded human liver tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
Background: | Acts as a transcription activator for MET and as a key regulator of HGF-MET signaling. Promotes cell motility, proliferation and hepatocyte growth factor (HGF)-dependent scattering in vitro and tumor growth and metastasis in vivo. |
Synonyms: | Metastasis-associated in colon cancer protein 1 (SH3 domain-containing protein 7a5), MACC1 |
UniProt Protein Function: | MACC1: Acts as a transcription activator for MET and as a key regulator of HGF-MET signaling. Promotes cell motility, proliferation and hepatocyte growth factor (HGF)-dependent scattering in vitro and tumor growth and metastasis in vivo. |
UniProt Protein Details: | Chromosomal Location of Human Ortholog: 7p21.1 Cellular Component: cytoplasm; nucleus Molecular Function:growth factor activity Biological Process: regulation of transcription, DNA-dependent; transcription, DNA-dependent; positive regulation of cell division |
NCBI Summary: | MACC1 is a key regulator of the hepatocyte growth factor (HGF; MIM 142409)-HGF receptor (HGFR, or MET; MIM 164860) pathway, which is involved in cellular growth, epithelial-mesenchymal transition, angiogenesis, cell motility, invasiveness, and metastasis. Expression of MACC1 in colon cancer (MIM 114500) specimens is an independent prognostic indicator for metastasis formation and metastasis-free survival (Stein et al., 2009 [PubMed 19098908]).[supplied by OMIM, Mar 2009] |
UniProt Code: | Q6ZN28 |
NCBI GenInfo Identifier: | 157502191 |
NCBI Gene ID: | 346389 |
NCBI Accession: | NP_877439.3 |
UniProt Secondary Accession: | Q6ZN28,Q6ZQN8, Q7Z5A5, A8MUS5, B2RNR9, |
UniProt Related Accession: | Q6ZN28 |
Molecular Weight: | 96,639 Da |
NCBI Full Name: | metastasis-associated in colon cancer protein 1 |
NCBI Synonym Full Names: | metastasis associated in colon cancer 1 |
NCBI Official Symbol: | MACC1 |
NCBI Official Synonym Symbols: | 7A5; SH3BP4L |
NCBI Protein Information: | metastasis-associated in colon cancer protein 1; putative binding protein 7a5; SH3 domain-containing protein 7a5 |
UniProt Protein Name: | Metastasis-associated in colon cancer protein 1 |
UniProt Synonym Protein Names: | SH3 domain-containing protein 7a5 |
Protein Family: | Metastasis-associated in colon cancer protein |
UniProt Gene Name: | MACC1 |
UniProt Entry Name: | MACC1_HUMAN |
Secondary Antibody |
Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014) |
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