Luciferase Firefly Recombinant Protein (RPPB1914)

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SKU:
RPPB1914
Product type:
Recombinant Protein
Size:
20ug
Target:
Luciferase Firefly
Synonyms:
Luciferase-like monooxygenase
Synonyms:
LUC
Synonyms:
EC 113127
Source:
Escherichia Coli
Uniprot:
P08659
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Description

Product Name:Luciferase Firefly Recombinant Protein
Product Code:RPPB1914
Size:20µg
Target:Luciferase Firefly
Synonyms:Luciferase-like monooxygenase, LUC, EC 1.13.12.7.
Source:Escherichia Coli
Physical Appearance:Sterile filtered colorless solution.
Formulation:The Luciferase protein solution (1mg/ml) contains 20mM Tris-HCl buffer (pH-8), 1mM DTT, and 10% glycerol.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Purity:Greater than 90.0% as determined by SDS-PAGE.
Amino Acid Sequence:MGSSHHHHHH SSGLVPRGSH MMEDAKNIKK GPAPFYPLED GTAGEQLHKA MKRYALVPGT IAFTDAHIEV DITYAEYFEM SVRLAEAMKR YGLNTNHRIV VCSENSLQFF MPVLGALFIG VAVAPANDIY NERELLNSMG ISQPTVVFVS KKGLQKILNV QKKLPIIQKI IIMDSKTDYQ GFQSMYTFVT SHLPPGFNEY DFVPESFDRD KTIALIMNSS GSTGLPKGVA LPHRTACVRF SHARDPIFGN QIIPDTAILS VVPFHHGFGM FTTLGYLICG FRVVLMYRFE EELFLRSLQD YKIQSALLVP TLFSFFAKST LIDKYDLSNL HEIASGGAPL SKEVGEAVAK RFHLPGIRQG YGLTETTSAI LITPEGDDKP GAVGKVVPFF EAKVVDLDTG KTLGVNQRGE LCVRGPMIMS GYVNNPEATN ALIDKDGWLH SGDIAYWDED EHFFIVDRLK SLIKYKGYQV APAELESILL QHPNIFDAGV AGLPDDDAGE LPAAVVVLEH GKTMTEKEIV DYVASQVTTA KKLRGGVVFV DEVPKGLTGK LDARKIREIL IKAKKGGKIA V

Luciferase is a general term for the class of oxidative enzymes used in bioluminescence and is distinct from a photoprotein. Luciferase catalyzes a bioluminescent reaction which involves the substrate luciferin as well as Mg2+ and ATP, produces green light with a wavelength of 562 nm. Luciferase from firefly is broadly used as a reporter for studying gene regulation and function, and for pharmaceutical screening.

Luciferase produced in E.Coli is a single, non-glycosylated polypeptide chain containing 571 amino acids (1-550 a.a.) and having a molecular mass of 62.9kDa.Luciferase is fused to a 21 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

UniProt Protein Function:Produces green light with a wavelength of 562 nm.
UniProt Protein Details:
NCBI Summary:
UniProt Code:P08659
NCBI GenInfo Identifier:126501
NCBI Gene ID:
NCBI Accession:P08659.1
UniProt Secondary Accession:P08659,Q27755
UniProt Related Accession:P08659
Molecular Weight:60,745 Da
NCBI Full Name:Luciferin 4-monooxygenase
NCBI Synonym Full Names:
NCBI Official Symbol:
NCBI Official Synonym Symbols:
NCBI Protein Information:
UniProt Protein Name:Luciferin 4-monooxygenase
UniProt Synonym Protein Names:
Protein Family:
UniProt Gene Name:Luciferase  
UniProt Entry Name:
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