LDH Cytotoxicity Assay Kit (CV0020)

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SKU:

CV0020

Product Type:

Assay

Research Area:

Cell Health

Description
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Description

LDH Cytotoxicity Assay Kit

LDH Cytotoxicity Assay Kit offers a Sensitive, Reproducible & Easy-to-use Colorimetric method for Cytotoxicity Detection.

Lactate dehydrogenase (LDH) is a ubiquitous enzyme present in cytosol of a wide variety of organisms. Since LDH is a fairly stable enzyme, it has been widely used to evaluate the presence of damage and toxicity of tissue and cells. Quantification of LDH has a broad range of applications. The measurement of this enzyme can be useful for estimating biomass, counting cells, and determining the health of cells and tissues. LDH is widely used for cytotoxicity testing of cultured cells as well as viability testing.

This assay kit uses the formazan dye INT as a colorimetric indicator of enzyme activity. LDH strips an electron from lactate which is transferred to NAD to yield NADH. The NADH subsequently reduces the INT which causes a color change from yellow to red. This colorimetric reaction can be measured at 490 nm.

Kit Information:

Overview	Description
Size:	400/2,000 Assays
Features & Benefits:	Ideal for High-Throughput Screening. Colorimetric: measures LDH release by formation of colored product. Fast and Easy-to-use: simple one-step procedure. Powerful: stable LDH enzyme activity as a Cytotoxic Marker. Safe: Non-radioactive.
Kit Contents (400 Assays):	20 mL LDH Assay Buffer 4 mL Lysis solution 20 mL Stop solution
Applications:	For rapid determination of Cytotoxicity based on lactate dehydrogenase released into cell culture medium. Evaluation of toxic compounds, toxins, detergents, environmental pollutants and physical treatment on cell lysis.
Shipping & Storage:	Shipped in: Gel Pack. Storage: LDH Assay Buffer (-20 °C) and Lysis Solution & Stop solution (+4°C). Recommendations: Protect the product from light. Shelf Life: 24 months from the date of purchase, if it is properly stored.
Safety:	This product is developed, designed and sold *exclusively for Research purposes and in vitro* use only (RUO)**. The product was not tested for use in diagnostics or for drug development, nor is it suitable for administration to humans or animals. For more info, please check its Material Safety Data Sheet available in this website.