Kinase Assay Kit (Fluorometric) (BA0125)
- SKU:
- BA0125
- Product Type:
- Assay
- Detection Method:
- Fluorometric
- Instrument:
- Microplate Reader
- Sample Type:
- All Kinases
- Research Area:
- Enzyme Activity
- High-Throughput Screening
Description
Kinase Assay Kit
Kinases , also known as phosphotransferases, constitute a large family of enzymes that transfer phosphate groups from the high-energy donor molecule ATP, to their specific substrates. Kinases are known to regulate the majority of cellular processes. The largest group of this family is the protein kinases. So far, 518 different kinases have been identified in humans and up to 30% of human proteins are modified by these kinases. The enormous diversity and their key role in cellular signaling make them ideal targets for drug developments.
Assay Genie's Kinase Assay Kit provides a simple and rapid method for assaying kinase activity and high-throughput screening for kinase inhibitors. This homogeneous microplate-based assay involves incubating the kinase with a single working reagent, in which ADP is enzymatically converted to ATP and pyruvate, which is quantified using a fluorometric (530nm/590nm) assay method.
Applications
Rapid homogenous assay for protein kinase activity and high-throughput screen for kinase inhibitors.
Key Features
- Safe. Non-radioactive assay.
- Sensitive and accurate. As low as 0.01 U/L kinase can be quantified.
- Homogeneous and convenient. "Mix-incubate-measure" type assay. The whole assay involves adding a single working reagent and incubation for 10 min at room temperature.
- Robust and amenable to HTS: Assay can tolerate up to 300 uM ATP and 10% dimethylsulfoxide (DMSO). Z' factors of > 0.6 are routinely observed in 96/384-well plates. Can be readily automated on HTS liquid handling systems for tens of thousands of assays per day.
Kinase Assay Kit Information:
| Kit Includes: | Reagent A: 10 mL Reagent B: 10 mL Assay Buffer: 25 mL Standard: 100 mL 3 mM ADP |
| Method of Detection: | FL530/585nm |
| Detection Limit: | 0.01 U/L |
| Samples: | All kinases |
| Species: | All |
| Protocol Length: | 10 min |
| Size: | 400 tests |
| Storage: | Store all reagents at -20°C |
| Shelf Life: | 6 months |
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