Isocitrate Dehydrogenase Assay Kit (BA0038)

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SKU:
BA0038
Product Type:
Assay
Instrument:
Microplate Reader
Sample Type:
Plasma, Serum, Tissue, Culture Media
Research Area:
TCA Cycle
Lipid Metabolism
Diabetes & Obesity
Plant & Environmental Stress
Enzyme Activity
Clinical Chemistry
Frequently bought together:

Description

ELISA Kit Technical ManualMSDS

Isocitrate Dehydrogenase Assay Kit - Information

This kit measures the activity of the NADP+ isoforms via Isocitrate Dehydrogenase Assay . Mutations in IDH1 and IDH2 have been linked with various brain tumors and acute myeloid leukemia. Assay Genie' non-radioactive, colorimetric IDH assay is based on the reduction of the tetrazolium salt MTT in a NADPH-coupled enzymatic reaction to a reduced form of MTT which exhibits an absorption maximum at 565 nm. The increase in absorbance at 565 nm is directly proportional to the enzyme activity.

Applications

For quantitative colorimetric kinetic determination of isocitrate dehydrogenase activity and evaluation of drug effects on its metabolism.

Isocitrate Dehydrogenase Assay Kit - Key Features

  • Fast and sensitive. Linear detection range (20 uL sample): 0.1 to 100 U/L for 30 min reaction.
  • Convenient and high-throughput. Homogeneous "mix-incubate-measure" type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day
Isocitrate Dehydrogenase Assay Kit - Data Sheet
Method of Detection OD565nm
Detection Limit 0.1 U/L
Samples Plasma, serum,tissue and culture media etc
Species All
Protocol Length 30 min
Size 100 tests
Shelf Life 6 months

More Details

ISOCITRATE DEHYDROGENASE (IDH) is an enzyme which catalyzes the interconversion of isocitrate and a-ketoglutarate. There are three IDH isoforms: IDH3 uses the cofactor NAD+ and catalyzes the third step in the citric acid cycle, while IDH1 and IDH2 use the cofactor NADP+ and catalyze the same reaction outside the citric acid cycle

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