Invertase Activity Assay Kit (BA0123)
- SKU:
- BA0123
- Product Type:
- Assay
- Instrument:
- Microplate Reader
- Sample Type:
- Biological, Environment (Soil)
- Research Area:
- Plant & Environmental Stress
- Enzyme Activity
Frequently bought together:
Description
Invertase Activity Assay Kit
Invertase (b-fructofuranosidase, EC 3.2.1.26) is an enzyme that catalyzes the hydrolysis of sucrose to fructose and glucose. Invertases cleave at the O-C(fructose) bond, whereas a related enzyme sucrase (EC 3.2.1.48) cleaves at the O-C(glucose) bond. A wide range of microorganisms produce invertase and can, thus, utilize sucrose as a nutrient. Invertase assay finds wide applications in environmental (e.g. soil), agricultural and food (confectionery) industry.
Assay Genie's Invertase activity Kit provides a convenient and ultra-sensitive colorimetric and fluorimetric means to measure invertase activity. In the assay, invertase cleaves sucrose, resulting in the formation of fructose and glucose, which is determined by a colorimetric (570nm) or fluorimetric method (Ex/Em = 530/585nm). The assay is simple, sensitive, stable and high-throughput adaptable.Applications
For quantitative determination of invertase/sucrase activityKey Features
- Safe. Non-radioactive assay.
- Sensitive and accurate. As low as 0.007 U/L invertase activity can be quantified.
- Homogeneous and convenient. "Mix-incubate-measure" type assay. No wash and reagent transfer steps are involved.
- Robust and amenable to HTS: can be readily automated on HTS liquid handling systems for processing thousands of samples per day.
Invertase Activity Assay Kit Information:
| Kit Includes: | 10x Reaction Buffer: 12 mL (pH 4.5), 10x Sucrose: 1.5 mL, Assay Buffer: 10 mL, Enzyme Mix: 120 mL Glucose Standard: 1 mL Dye Reagent: 120 mL |
| Kit Requires: | Pipetting devices, centrifuge tubes, clear or black flat bottom 96-well plate. |
| Method of Detection: | OD570nm, or FL530/585nm |
| Detection Limit: | 0.007 U/L |
| Samples: | Biological, environment (soil) etc |
| Species: | All |
| Protocol Length: | 40 min |
| Size: | 100 tests |
| Storage: | Store Reaction Buffer and Assay Buffer at 4°C and other reagents at - 20°C. |
| Shelf Life: | 6 months |
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