Hydrogen Peroxide Assay Kit (BA0047)

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SKU:
BA0047
Product Type:
Assay
Instrument:
Microplate Reader
Sample Type:
Serum, Citrate-Plasma, Urine, Cell Lysate, Culture Media
Research Area:
Plant & Environmental Stress
Frequently bought together:

Description

ELISA Kit Technical ManualMSDS

Hydrogen Peroxide Assay Kit - Information

Assay Genie's hydrogen peroxide assay kit is designed to measure peroxide concentration in biological samples without any pretreatment. The improved method utilizes the chromogenic Fe3+-xylenol orange reaction, in which a purple complex is formed when Fe2+ provided in the reagent is oxidized to Fe3+ by peroxides present in the sample. The intensity of the color, measured at 540-610nm, is an accurate measure of the peroxide level in the sample. The optimized formulation substantially reduces interference by substances in the raw samples.

Applications

For quantitative determination of peroxide and evaluation of drug effects on hydrogen peroxide metabolism.

Hydrogen Peroxide Assay Kit - Key Features

  • Sensitive and accurate. Enhanced color intensity using sorbitol. Detection range 0.2 (7 ng/mL) to 30 (1,020 ng/mL) hydrogen peroxidein 96-well plate assay.
  • Simple and high-throughput. The procedure involves addition of a single detection reagent and incubation for 30 min. Can be readily automated as a high-throughput assay in 96-well plates for thousands of samples per day.

Hydrogen Peroxide Assay Kit - Data Sheet

Kit IncludesReagent A: 1 mL Reagent B: 50 mL Standard: 100 mL 3% stabilized H2O2.
Kit RequiresPipetting devices and accessories, 96-well plates and plate reader.
Method of DetectionOD585nm
Detection Limit0.2
SamplesSerum, citrate-plasma, urine, cell lysate, culture media etc
SpeciesAll
Protocol Length30 min
Size250 tests
StorageStore all reagents at 4 °C
Shelf Life6 months

More Details

Peroxide (e.g. hydrogen peroxide H2O2) is one of the key reactive oxygen species formed under oxidative stress conditions. High levels of peroxide formation have been linked to pathological conditions such as ageing, asthma, diabetes, atherosclerosis, cataract, inflammatory arthritis and neurodegenerative diseases. Simple, direct and automation-ready procedures for quantitative determination of peroxide find wide applications in research and drug discovery.

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